Page 8 - IJA-Vol.3-No.23
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International Journal of Aquaculture, 2013, Vol.3, No.23, 133
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137
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meal, maize, fish meal, vitamins premix, di-calcium
phosphate, and salt purchased and milled at Bodija
Market, Ibadan. The 50% C.P was based on the
protein requirement of
O. niloticus
fry (30-56) % as
recommended by Jauncey (2000). The milled
feedstuffs were passed through a 200 μm sieve mesh.
3.3
Experimental Procedures
The experiment was a completely randomized design
with six treatments and three replicates respectively
representing different inclusion rate of 0.0 g, 1.0 g, 1.5 g,
2.0
g, and 2.5 g
T. terrestris
extract to 1 kg basal diet
(
T1-5) treatment 6 containing 50 mg/kg of
17-
α-methyltestosterone to basal diet as control. 450
day-old fry of
O. niloticus
were randomly distributed
into 30L capacity plastic aquaria and were
acclimatized for 3 days before the commencement of
feeding with experimental diets in the laboratory.
Each aquarium was adequately aerated. The fry were
fed with the diet
ad libitum
from day four thrice daily
for 42. Settled fish wastes were siphoned with half of
aquarium's water, fresh aerated water was used to
replenish daily while the water in the aquaria were
changed completely twice a week. At the end of
reversal stage, the fish were transferred into the
Departmental fish farm into hapas rigged in an earthen
pond and were fed for 70 days with commercial diet.
The fish were weighed biweekly and water quality
parameters monitored. The fish were sexed on the
70
th day using a hand binocular. The following
growth performance parameters were determined;
Mean Weight Gain, Length of fish, Food Conversion
Ratio and Specific Growth Rate. Growth performance
was determined and feed utilization was calculated
according to Ogunji et al. (2000) andAhmad et al. (2002).
Water quality parameters (temperature, dissolved
oxygen, pH, ammonia and nitrite) were determined
weekly. Dissolved oxygen, Ammonia and Nitrite were
measured using Hach’s Model FF-1A Fish Farmer’s
Water Quality Test Kit. Temperature was measured
using a mercury-in-glass thermometer. The pH was
monitored using Hanna model H1-98107.
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