Table of Contents - MPBv3no4

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Table of Contents

Research Report

Over Expression of Chitinase and Chitosanase Genes from Trichodermaharzianum under Constitutive and Inducible Promoters in order to IncreaseDisease Resistance in Wheat (Triticum aestivum L)

Introduction

1.1 Genetic transformation of wheat

1.2 Molecular verification of pUbi-Harchit andpUbi-Harcho transgenic plants and their progenies

1.3 Molecular verification of pVst-HarChit andpVst-HarCho transgenic plants

1.4 Molecular verification of transgenes in segregatinggeneration

1.5 Expression analysis of pUbi-HarChit andpUbi-HarCho transgenes by Northern Blot

1.6 Induction of HarChit and HarCho genes underthe control of Vst1 promoter

1.7 Phyto-pathological experiments

1.8 Transgenic lines under constitutive promoter

2 Discussions

2.1 Wheat transformation, transformation frequencyand segregation in the progenies

2.2 Co-expression of HarChit and HarCho

2.3 Performance of transgenics under constitutivepromoter

2.4 Performance of transgenics under induciblepromoter

3 Materials and Methods

3.1 Wheat transformation

3.2 Expression vector construction

3.3 Molecular analysis of putative transgenics

3.4 DNA isolation and Southern Blot analysis

3.5 RNA isolation and Northern Blot/RT-PCR

3.6 Inoculation with Erysiphe graminis f sp. tritici

3.7 Detached leaves infections

Acknowledgements