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Medicinal Plant Research 2012, Vol.2, No.2, 6
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adsorption, correspondingly. After 48 h incubation at
37
℃
, virus titer was determined by the endpoint
dilution method and expressed as TCID50 /mL.
EC50, the concentration needed to restrain 50% virus
infection compared to untreated infected cells, were
determined directly from the curve obtained by
plotting the inhibition of the virus yield against the
concentration of the samples.
3.6 Statistical analysis
The mean number of viral plaques of two different
experiments was compared with oneway analysis of
variance (ANOVA) using SPSS v11.5. Dunnett test
was used as the post hoc test. A P<0.05 was consi-
dered statistically significant.
References
Batish D.R., Singh H.P., Kohli R.K., and Kaur S., 2008, Eucalyptus
essential oil as a natural pesticide, Forest Ecology and Management,
256(12): 2166-2174 http://dx.doi.org/10.1016/j.foreco.2008.08.008
Carson C.F., Smith D.W., Lampacher G.J., and Riley T.V., 2008, Use of
deception to achieve double-blinding in a clinical trial of Melaleuca
alternifolia (tea tree) oil for the treatment of recurrent herpes labialis,
Contemporary Clinical Trials, 29(1): 9-12 http://dx.doi.org/10.1016/
j.cct.2007.04.006
Chattopadhyay D., and Khan M.T.H., 2008, Ethnomedicines and
ethnomedicinal phytophores against herpesviruses, in: El-Gewely M.R.
(Ed.), Biotechnology Annual Review, Elsevier, pp.297-348
Chuanasa T., Phromjai J., Lipipun V., Likhitwitayawuid K., Suzuki M.,
Pramyothin P., Hattori M., and Shiraki K., 2008, Anti-herpes simplex
virus (HSV-1) activity of oxyresveratrol derived from Thai medicinal
plant: Mechanism of action and therapeutic efficacy on cutaneous
HSV-1 infection in mice, Antiviral Research, 80(1): 62-70 http://dx.
doi.org/10.1016/j.antiviral.2008.05.002
El Sayed K.A., 2000, Natural products as antiviral agents, in: R. Atta ur
(Ed.), Studies in Natural Products Chemistry, Elsevier, pp.473-572
Fatahzadeh M., and Schwartz R.A., 2007, Human herpes simplex virus
infections: Epidemiology, pathogenesis, symptomatology, diagnosis,
and management, Journal of the American Academy of Dermatology,
57(5): 737-763 http://dx.doi.org/10.1016/j.jaad.2007.06.027
Friedman H.M., 2006, Keratin, a dual role in herpes simplex virus
pathogenesis, Journal of Clinical Virology, 35(1): 103-105 http://dx.doi.
org/10.1016/j.jcv.2005.03.008
Garozzo A., Timpanaro R., Stivala A., Bisignano G., and Castro A., 2011,
Activity of melaleuca alternifolia (tea tree) oil on Influenza virus
A/PR/8: Study on the mechanism of action, Antiviral Research, 89(1):
83-88 http://dx.doi.org/10.1016/j.antiviral.2010.11.010
Gebre-Mariam T., Neubert R., Schmidt P.C., Wutzler P., and Schmidtke M.,
2006, Antiviral activities of some ethiopian medicinal plants used for
the treatment of dermatological disorders, Journal of Ethnopharma-
cology, 104(1-2): 182-187 http://dx.doi.org/10.1016/j.jep.2005.08.071
Gershon A.A., Gershon M.D., Breuer J., Levin M.J., Oaklander A.L., and
Griffiths P.D., 2010, Advances in the understanding of the pathogenesis
and epidemiology of herpes zoster, Journal of Clinical Virology, 48(S1):
2-7 http://dx.doi.org/10.1016/S1386-6532(10)70002-0
Hammer K.A., Carson C.F., Riley T.V., and Nielsen J.B., 2006, A review of
the toxicity of melaleuca alternifolia (tea tree) oil, Food and Chemical
Toxicology, 44(5): 616-625 http://dx.doi.org/10.1016/j.fct.2005.09.001
Hosono T., Yokomizo K., Hamasaki A., Okamoto Y., Okawara T., Otsuka
M., Mukai R., and Suzuki K., 2008, Antiviral activities against herpes
simplex virus type 1 by HPH derivatives and their structure-activity
relationships, Bioorganic & Medicinal Chemistry Letters, 18(1):
371-374 http://dx.doi.org/10.1016/j.bmcl.2007.10.065
Ju H.Q., Wang S.Y., Pei Y., Xiang Y.F., Li S., Zhang Y.J., Yang C.R., and
Wang Y.F., 2011,
In vitro
study on the anti-HSV-1 and HBV activities
of extracts from the fruit of Eucalyptus maidenii, Zhong Yao Cai, 34(2):
242-245
Khan M.T.H., Ather A., Thompson K.D., and Gambari R., 2005, Extracts
and molecules from medicinal plants against herpes simplex viruses,
Antiviral Research, 67(2): 107-119 http://dx.doi.org/10.1016/j.antiviral.
2005.05.002
Knickelbein J.E., Hendricks R.L., and Charukamnoetkanok P., 2009,
Management of herpes simplex virus stromal keratitis: an evidence-
based review, Survey of Ophthalmology, 54(2): 226-234 http://dx.doi.
org/10.1016/j.survophthal.2008.12.004
Koch C., Reichling J., Schneele J., and Schnitzler P., 2008, Inhibitory effect
of essential oils against herpes simplex virus type 2, Phytomedicine,
15(1-2): 71-78 http://dx.doi.org/10.1016/j.phymed.2007.09.003
Roller D.G., Dollery S.J., Doyle J.L., and Nicola A.V., 2008, Structure-
function analysis of herpes simplex virus glycoprotein B with fusion-
from-without activity, Virology, 382(2): 207-216 http://dx.doi.org/10.
1016/j.virol.2008.09.015
Wei A., and Shibamoto T., 2010, Chapter 4-Medicinal activities of essential
oils: role in disease prevention, in: Ronald Ross W. and Victor R.P.
(Eds.), Bioactive Foods in Promoting Health, Academic Press, San
Diego, pp.59-70 http://dx.doi.org/10.1016/B978-0-12-374628-3.00004-9
Xu H.X., Lee S.H.S., Lee S.F., White R.L., and Blay J., 1999, Isolation and
characterization of an anti-HSV polysaccharide from Prunella vulgaris,
Antiviral Research, 44(1): 43-54 http://dx.doi.org/10.1016/S0166-3542
(99)00053-4
Yang K., Wills E.G., and Baines J.D., 2012, Release of the herpes simplex
virus 1 protease by self cleavage is required for proper conformation of
the portal vertex, Virology, 429(1): 63-73 http://dx.doi.org/10.1016/j.
virol.2012.03.009