Page 19 - 2011no56

Basic HTML Version

分子植物育种
(
网络版
), 2011
,
9
,
1418
-
1424
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1418
-
1424
http://mpb.
5th
.sophiapublisher.com
1418
研究报告
A Letter
洋葱种质资源遗传多样性的
SSR
分析
关绚丽
1,2
,
田保华
1,3
,
梁毅
1
,
王永勤
1
1.
北京市农林科学院蔬菜研究中心
,
北京
, 100097
2.
山西农业大学园艺学院
,
晋中
, 030801
3.
山西大学生命科学学院
,
太原
, 030005
通讯作者
:
liangyi222@yahoo.com.cn;
作者
分子植物育种
, 2011
,
9
,
56
doi: 10.5376/mpb.cn.2011.09.0056
收稿日期:
2011
03
22
接受日期:
2011
04
28
发表日期:
2011
05
11
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式:
关绚丽等
, 2011,
洋葱种质资源遗传多样性的
SSR
分析
,
分子植物育种
Vol.9 No.56 (doi: 10.5376/mpb.cn.2011.09.0056)
本研究利用
SSR
标记对
63
份洋葱种质资源遗传多样性进行了分析,以期为洋葱亲本选配提供参考。研究结果表
明:从
40
SSR
引物中筛选出
8
对扩增条带清晰、可重复、多态性高的引物,在
63
份资源样品中共扩增出
50
条带,其
中检测到
27
个等位变异,多态性条带比率
(PPB)
54%
,每对
SSR
引物检测到等位变异
2~5
个,平均
3.125
个。
8
对引物
的多态性信息量
(PIC)
变化范围为
0.225 4~0.698 7
,多态性信息量
(PIC)
Shannon
信息指数
(I)
Nei's
基因多样性指数
(H)
平均分别为
0.497 3
0.904 1
0.520 0
UPGMA
聚类分析表明,
SSR
标记可以将
63
份洋葱品种分为
2
大类:
A
类为短日
照洋葱群与中日照早熟洋葱群;
B
类为长日照洋葱群与中日照晚熟洋葱群,长日照洋葱群又分别包括
Spanish
长日照洋葱
Yellow Globe Danvers
耐贮性长日照洋葱;结果也显示了中日照洋葱起源的复杂性。
关键词
洋葱
; SSR;
遗传多样性
Genetic Diversity Analysis of Onion (
Allium cepa
L.) Germplasm by SSR
Markers
Guan Xuanli
1,2
, Tian Baohua
1,3
, Liang Yi
1
, Wang Yongqin
1
1. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, P.R. China
2. Horticultural College, Shanxi Agricultural University, Jinzhong Shanxi, 030801, P.R. China
3. School of Life Science, Shanxi University, Taiyuan, 030005, P.R. China
Corresponding author, liangyi222@yahoo.com.cn;
Authors
Abstract
In this paper, we analyzed the genetic diversity of 63 onions (
Allium cepa
L.) germplasm resources were analyzed by
SSR markers in order to provide reference for choice of parents for crossing in onion. The results showed that eight clear, repeatable,
highly polymorphic SSR primers were screened from 40 SSR primers, 50 clear bands were amplified from 63 resources, among
which detected 27 alleles, the polymorphism ratio of bands (PPB) was 54%, detected 2~5 alleles and averaged 3.125 bands each
primer pair. The polymorphism information content (PIC) ranged from 0.225 4 to 0.6 987, the average of polymorphism information
content (PIC), Shannon information index (I), Nei's genetic diversity index (H) was 0.497 3 , 0.904 1
0.520 0 respectively. UPGMA
cluster analysis indicated that the germplasms were both divided into two clusters base on SSR markers, cluster A was included
short-day onion group and early maturity of intermediate-day onion group, cluster B was included long-day onion group and late
maturity of intermediate-day group onion, and in the long-day group Spanish long-day onion and storage long-day type onion were
included, the result also expressed the originating complication of intermediate -day type onion.
Keywords
Onion; SSR; Genetic diversity
研究背景
洋葱
(
Allium cepa
L.)
又名球葱、圆葱、玉葱,
属于百合科葱属
(
Allium
)
二年生植物,世界上重要的
蔬菜之一,是中国主要栽培和出口的蔬菜之一
(Hou
et al., 1997)
。在全世界中,洋葱的种植面积仅次于
马铃薯和番茄,富含多种硫化物和糖类。具有降低
和预防血栓的形成,抗动脉硬化、降血脂、降血压、
预防心肌梗塞的作用,广泛应用于食品、医药和保
健等领域,随着进一步深入研究,洋葱已经成为一
种越来越重要的植物资源
(
刘冰江等
, 2007;
陈沁滨