International Journal of Marine Science 2015, Vol.5, No.53: 1-6
1
Research Report Open Access
Antibacterial Potential Screening of
Halimeda
sp on Some Types of Pathogenic
Bacteria
Hendri M.
1,2,
, Darmanto J. S.
3
, Prayitno B.
3
, Radjasa O.K.
4
1. Program Study of Marine Science, University of Diponegoro, Kampus Pleburan, Semarang,West Java, Indonesia
2. Program Study of Marine Science, University of Sriwijaya, Kampius Inderalaya, Ogan Ilir, South Sumatra, Indonesia
3. Program Study of Fisheries, University of Diponegoro, Kampus Tembalang, Semarang,West Java, Indonesia
4. Program Study of Marine Science, University of Diponegoro, Kampus Tembalang, Semarang,West Java, Indonesia
Corresponding author email
International Journal of Marine Science, 2015, Vol.5, No.53 doi
Received: 07 May, 2015
Accepted: 08 Aug, 2015
Published: 17 Sep., 2015
Copyright
©
2015 Hendri et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article: Hendri M.1,, Darmanto J. S., Prayitno B. and Radjasa O.K., 2015, Antibacterial Potential Screening of
Halimeda
sp on Some
Types of Pathogenic Bacteria, International Journal of Marine Science, 5(53): 1-6
Abstract
The study was conducted as a test to determine the effectiveness of
Halimeda
sp seaweed extract on the growth of some
types of pathogenic bacteria. Seaweeds extracted consist of four (4) types which include:
Halimeda macrophysa
,
Halimeda gracillis
,
Halimeda Opuntia
and
Halimeda renschi
. While the types of pathogenic bacteria used were (
Salmonella typhi
,
Staphylococcus
aureus, Escherichia coli
and
Bacillus subtilis
). This study uses methanol in the ratio of 1: 1 (v/v) and were observed for 48 hours.
The test results showed that the extract of
Halimeda
sp is effective as antibacterial pathogen. Phytochemical test showed the presence
of steroid and saponin compounds.
Keywords
Antibacterial; Bacterial Pathogens;
Halimeda
sp; Seaweed
1 Introduction
Seaweed is one of important marine commodities that
have high economic value for export. Currently
seaweed has been developed by means of cultivation.
This activityy is carried out by various parties such as
companies, governments, and fishermen community.
The benefits of this plant are commonly known as
product of food, beauty, medicines and others (Aslan,
1998; Anggadireja et al., 2006).
Some marine organisms, especially from the class of
marine algae, have the ability to produce chemical
compounds that are not found or rarely found in
organisms that live on land (Nybakken, 1993). Some
types of marine biota synthesize and store toxic
compounds called marinetoxin on parts of his body and
released into the environment (Djapiala et al., 2013;
Anggadireja et al., 2006). These compounds are
secondary metabolites which are used as a defense
and to preserve life, to avoid interference from
predators. These compounds have pharmacological
activity, so it is possible to be developed (Paul and
Fenical, 1983; Paul and Puglisi, 2004; Paul and
Fenical, 1984; Paul and Van Alstyne, 1988).
Halimeda is a marine plant that has green leaves and is
one type of green algae group. Halimeda has the ability
to produce bioktif substances for antifouling. The
active substance produced for biofouling is known as
halimedatrial
and
halimedatetraasetat
.
Halimedatrial
is
diterpenoid
that yet trialdehyde, known as the major
secondary metabolite in six species of algae containing
calcium Halimeda (Paul and Fenical, 1983; Paul and
Fenical, 1984; Paul and Puglisi, 2004; Kumar et al.,
2010; Bachtiar et al., 2012; Paul, 1987).
Seaweed, primarily from the group
Halimeda
sp has
the ability to issue a secondary metabolite in the
process of metabolism to defend themselves against
predators and pests. The active ingredients released by
Halimeda are very effective to prevent attacks of
predators and bacteria (antifouling). Halimedatrial and
halimedatetraasetate a bioactive compounds contained
in seaweed (
Halimeda
sp) (Paul and Fenical, 1983;
Paul and Fenical, 1984; Paul and Fenical, 1986; Paul,
1987; Atmadja, 1992; Paul and Van Alstyne, 1992).
The ability of algae to produce halogenated secondary
metabolites that act as bioactive compounds might
happen, because the environmental conditions such as
high salinity or will be used to defend themselves
from the threat of predators. In the last decade, a
variety of structures of bioactive compounds that very
unique from red algae have been isolated. However,
