Cotton Genomics and Genetics 2015, Vol.6, No.4, 1-7
6
At the time of flowering direct crosses were made.
Emasculation was done at evening and pollination was
performed next morning at 9 to 10 am. In June 2013,
F
0
seed of 10 hybrids and 5 parents were planted in
the field in randomized complete block design with
three replications. In each replication there was single
row for each hybrid and parent. Row-to-row and
plant-to-plant distance was maintained 75cm and
30cm, respectively. Proper agronomic practices were
adopted from sowing till harvesting of crop. Five
plants were selected from each parent for recording of
data. Data was recorded for following polygenic traits:
plant height, number of sympodial branches, number
of monopodial branches, number of bolls, fibre length,
fibre strength, fibre fineness, ginning-out-turn, weight
of 100 seeds and seed cotton yield.
After harvesting, ginning was performed with help of
ginner, in department of Plant Breeding and Genetics.
Weight of randomly selected 100 seeds was measured
on electrical balance. Ginning out trun (GOT) was
calculated by formula:
Fibre related characters - fibre length, fibre strength
and fibre fineness- were calculed by help of Zellwegar
Uster HVI-900 in the Department of Fibre Technolog,
University of Agriculture, Faisalabad. Data of fibre
length, fibre strength and fibre fineness was recorded
in millimeter, gram per tax and microgram per inch.
Analysis of Data:
Data was subjected to analysis of variance by procedure
given by Steel and Torrie (1987) for determining
significant differences among the genotypes under
study. Correlation analysis- phenotypic and genotypic
correlation- and Path coefficient analysis was done by
procedure outlined by Dewey and Lu (1959) and path
coefficient analysis was done by using genotypic
correlation. Broad sense heritability was determined
according to formula of Mahmud and Kramer (1951)
as follows:
Genetic advance calculated according to prescribed
formula of Allard (1960).
G.A = σ ρ× h
2
×i
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