Page 7 - ME-436-v3-3

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Journal of Mosquito Research, 2013, Vol.3, No.11, 76-81
ISSN 1927-646X
http://jmr.sophiapublisher.com
78
Table 2
Completely randomized three-way factorial ANOVA using different concentrations, period of exposure and different instar as
variables
Source of variation
Sum of squares
df
Mean square
F value
P value
Hours (H)
38.23
2
19.11
66.58
0.003
Instar (I)
85.37
3
28.46
99.14
0.004
Concentration(C)
68.72
2
34.36
119.71
0.005
H × I
1.18
6
0.11
0.69
0.660
H × C
8.22
4
2.05
7.16
0.001
I × C
17.35
6
2.89
10.07
0.002
H × I × C
3.25
12
0.27
0.95
0.507
Residual
20.67
72
0.29
Total
243.00
107
Table 3
Log probit analysis and regression analysis of larvicidal activity of
crude extracts of seed coats of
C.
sophera
against all
instar larvae of
Cx. quinquefasciatus
Larval instars
Period of bioassay(h) LC
50
LC
90
Regression equation
R value
24
0.60
1.02
Y=16x+2
0.49
48
0.54
0.78
Y=16x+4.86
0.71
1
st
72
0.50
0.72
Y=10.667x+9.86
0.68
24
0.83
2.41
Y=12.667x-0.66
0.52
48
0.42
1.81
Y=7.6667x+7.93
0.45
2
nd
72
0.42
1.19
Y=9.3333x+8
0.57
24
1.13
5.36
Y=8x+1.26
0.46
48
0.47
1.17
Y=11.333x+6.06
0.49
3
rd
72
0.32
0.79
Y=6.6667x+12.46
0.54
24
1.25
6.54
Y=7x+1.6
0.45
48
0.58
1.77
Y=11.667x+3.26
0.34
4
th
72
0.21
1.37
Y=5.6667x+11.93
0.34
Table 4
Result of larval mortality of different concentration of ethyl acetate solvent extract of
seed coat of
C.
sophera
on all instar of
Cx. quinquefasciatus
Larval instar
Concentration (ppm)
Mean mortality (%) ± SE
24 h
48 h
72 h
480
7.67 ± 0.33
9.33 ± 0.67
10.00 ± 0.00
500
8.67 ± 0.33
9.67 ± 0.33
10.00 ± 0.00
1
st
520
10.00 ± 0.00
10.00 ± 0.00
10.00 ± 0.00
480
7.33 ± 0.33
8.67 ±0.33
9.67 ± 0.33
500
8.33 ± 0.33
8.67 ±0.33
9.33 ± 0.33
2
nd
520
9.67 ± 0.33
10.00 ± 0.00
10.00 ± 0.00
480
5.33 ± 0.33
7.33 ± 0.33
8.00 ± 0.58
500
8.33 ± 0.33
8.67 ± 0.33
9.33 ± 0.33
3
rd
520
8.67 ± 0.33
9.00 ± 0.00
10.00 ± 0.00
480
4.33 ± 0.33
5.67 ± 0.33
6.33 ± 0.33
500
6.67 ± 0.33
7.67 ± 0.33
8.00 ± 0.00
4
th
520
7.67 ± 0.33
8.67 ± 0.33
9.33 ± 0.33