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Bt Research 2013, Vol.4 No.2 8-13
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12
described by Sambrook et al. (1989). PCR reactions
were performed in a MJ Research, Inc. (PTC-100TM)
thermal cycler and used 10 µL template DNA from
each sample 8.0 µM of each specific primer, 5 mM
dNTP mix, 10× Taq buffer and 2.5 U Taq DNA
polymerase in a reaction of 50 µL. The amplification
conditions were described by Bravo et al. (1998) and
Ibarra et al. (2003). After amplification, 20 μL of
each PCR product was loaded on a 1.5% agarose
gel stained with ethidium bromide and visualized
under ultraviolet light. The
Bti
strain T14001 was
used as standard.
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