Basic HTML Version
Molecular Microbiology Research (Online) 2013, Vol.3 No.2 9-20
ISSN 1027-5595
http://mmr.sophiapublisher.com
14
Continuing table 5
0.5 mL
1 mL
2.5 mL
5 mL
S.
No.
Food
types
Sample
category
No. of
colony
No. of
mutant
No. of
colony
No. of
mutant
No. of
colony
No. of
mutant
No. of
colony
No. of
mutant
Degree
of
mutagenicity (-), (1+)
and (2+)
Chicken II
257
239
19 291
71 316
96
1+
Fish I
285
233
13 311
91 261
41
1+
Fish II
255
250
30 308
88 248
28
1+
Sample I
231
251
31 240
20 238
18
1+
Sample II
292
278
58 268
48 299
79
1+
Sample III
298
301
81 281
61 319
99
1+
Sample IV
265
249
29 309
89 301
81
1+
7
Reheated
oil
(crude)
Sample V
253
291
71 249
29 256
36
1+
Orange colour
248
28 238
18 262
42
1+
Yellow colour
239
19 251
31 233
13
1+
Red colour I
251
31 247
27 239
19
1+
Red colour II
255
35 268
48 258
38
1+
8
Jangiri
Red colour III
240
20 261
41 231
11
1+
Green colour I
8
-
42
-
62
-
-
Green colour II
50
-
38
-
88
-
-
Yellow colour I
17
-
110
-
18
-
-
Yellow colour II
91
-
78
-
52
-
-
9
Milk
sweet
White colour
58
-
89
-
57
-
-
Sample I
330 110 420 200 337 117
2+
Sample II
239
19 249
29 300
80
1+
Sample III
239
18 231
11 249
29
1+
Sample IV
260
40 287
67 246
26
1+
10
Halwa
Sample V
292
72 271
51 255
35
1+
Red colour I
10
-
48
-
88
-
-
Yellow colour
231
11 241
21 281
61
1+
Green colour I
266
46 258
38 269
49
1+
Green colour II
253
33 267
47 247
27
1+
11
Kesari
Red colour II
249
29 279
59 298
78
-
Table 6 Antibiotic markers among
Salmonella typhimurium
strains
Treated strain MTCC 98
S. No.
Antibiotic used
UV
NTG
Auxotropic mutant
MTCC 1251
Wild type
1
Strapiomycin (100 mg /mL)
+
-
-
-
2
Tetracycline (25 mg/mL)
+
-
+
-
3
Ampicillin (mg/mL)
-
+
+
-
4
Kanomycin (25 mg/mL)
+
-
-
-
Salmonella typhimurium
MTCC 1251 and the treated
strains of MTCC 98 were tested for amino acid and
antibiotic markers (Table 6 and Table 7). In amino
acids marker study, markers for the tested 12 amino
acids were found to get transformed to the treated
strains within one hour of incubation. No difference in
amino acid markers were observed after 2 hrs and 3
hrs incubation studies. The auxotrophic mutant strain
of MTCC 1251 showed his+ and argi+ character.
There were no other notable changes.
Table 7 represents the antibiotic marker changes
among the strains used. Treated strains were formed to
receive the resistant marker for Str+, Tet+ and kan+
(within one hr incubation) while auxotrophic mutant
strain was able to develop resistance to tetracycline
and ampicillin during one hour incubation. The wild
type strain showed sensitivity towards all antibiotics.