Molecular Plant Breeding 2016, Vol.7, No.33, 1
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Figure 1 (a) Foreground analysis of advance backcross lines (ABLs) of Osmancik-97. DNA was amplified with 9871.T7E2b as a
primer for the
Pi40
gene. Arrows indicate resistance specific-alleles. Legend: Kb
+
= DNA ladder; P1=Osmancık-97;
P2=IR83260-1-1-1-5-B-3-1-2-B; 1-1=ABLs
Figure 1 (b) Applicability of
Pi40
marker to high throughput genotyping. The experiment was performed using Fragment Analyzer
following manufacturer’s instructions with 35 bp and 1,500 bp as upper and lower limits respectively. Resistant specific-alleles are
194 bp and 230 bp. BG=background
A total of 14 lines were processed for background genotyping which includes 11 ABLs, two recurrent parents
(Halilbey and Osmancik-97), and the DP by Illumina 6K Infinium single nucleotide polymorphism (SNP) Chip
from Cornell University was used in this study and was originally designed to contain a total of 5,274 SNPs. After
employing quality controls and clustering, a total of 4,606 SNPs were found to be of high quality. Comparing the
SNP genotypes of Halilbey and DP, 795 (17.26%) of the SNPs were found to be polymorphic between the parents.
