Molecular Plant Breeding, 2016, Vol.7, No.15, 1-12
1
Research Ariticle
Open Access
Development of Source Independent Micropropagation System in
Dalbergia
sissoo
Roxb, as a Basis for Germplasm Conservation and Disease Free Plants
Production
Shan-e-Ali Zaidi
1
, Siddra Ijaz
1
,
,
Azeem Iqba Khan
1,2
, Iqrar Ahmad Rana
1
1 Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Pakistan
2 Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan
Corresponding author email:
Molecular Plant Breeding 2016, Vol.7, No.15 doi:
Received: 10 Dec., 2015
Accepted: 19 Feb., 2016
Published: 19 Feb., 2016
Copyright
© 2016 This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Zaidi S.A., Ijaz S., Iqba A., Khan, and Rana I.A., 2016, Development of Source Independent Micropropagation System in
Dalbergia sissoo
Roxb, as a Basis for
Germplasm Conservation and Disease Free Plants Production, Molecular Plant Breeding,7(15):1-12 (doi:
)
Abstract
Plant micropropagation has becoming an imperative strategy at commercial level that provides large number of plants in
less time and space. In woody trees, micropropagation is more tedious and cumbersome process than other crop plants. Less success
rate in tree micropropagation has been reported in literature. Therefore in this study, expedite, reproducible and source independent
micropropagation protocol was developed in
Dalbergia sissoo
(Shisham) that is an economically important timer yielding tree with
medicinal values. For this purpose, eight micropropagation media were studied with various combination of BAP
(benzylaminopurine) and IAA (indole acetic acid). Auxially and epicormic shoots of
Dalbergia sissoo
having at least one nodal bud
were used as explants. We studied the impact of these media combination and explant types on micropropagation. We found MPMS5
is the best medium combination that gave maximum number of shoots (2.78 shoots per explant) followed by MPMS7. This selected
media combination can be used for
Dalbergia sissoo
micropropagation as a routine work. Similarly rooting media combinations were
also studied for the best root induction in micropropagated shoots of
Dalbergia sissoo
but little success had been achieved.
Keywords
Dalbergia sissoo
; Micropropagation; Shisham; Tissue culture; Woody tree
Introduction
Urbanization and deforestation are the pivotal forces
those bring negative stress to natural resources.
Evolution of problems relating to these forces is
basically due to rapid increase in human population.
Ecosystem in general and habitats of endangered
species in particular are affected by these situations.
Many industries depend upon the continuous supply
of wood, can not totally rely on natural sources,
therefore efforts should be done to find new ways to
fulfill their demand for timber (Jain, 1997 and Tzfira
et al., 1998). Conventional propagation through
cuttings and suckers do not provide the required plant
material to support the increasing timber demand due
to various factors (Ali et al., 2005). Therefore the
focuses should be on replacing these conventional
means with modern in-vitro techniques. Biotic and
abiotic factors also affect trees that result in declined
tree populations (Rehman et al., 2012). Development
of resistant material against various natural calamities
will reduce supply-demand strain and will be more
beneficial for farmers as well for industries (Minocha
and Jain, 2000). Different conventional and non
conventional strategies have been employed to
multiply the resistant plants in huge number (Pradhan
et
al.,
1998).
Micropropagation facilitates
multiplication from limited mother stock, keep
product uniformity, not dependent upon season and
many other agronomic advantages (Kunisaki et al.,
2003). Micro- propagation in economic tree species is
also now becoming an emerging tool and requirement
of this modern era.
Dalbergia sissoo
Roxb is a very important timber
yielding perennial tree found throughout IndoPak
subcontinent. It has been used for forestation in most
parts of the subcontinent for years (Priyanka et al.,
2012). It has great economic importance as its wood is
used in making furniture, agricultural tools, used as
fuel and also has some medicinal value. At least half a
