Legume Genomics and Genetics 2016, Vol.7, No.1, 1-11
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2.2 Identification of heat tolerant genotypes
In the present study, eight genotypes FLIP2009-55L
(43.7) IG2507 (43.7), IG3327 (43.3), IG 3330 (45.5),
IG 3546 (47.2), IG 3745 (51.2), IG 4258 (47.7) and IG
5146 (47.7) had significantly large number of filled
pods per plant at higher temperature (>35 ºC). Also,
genotypes, namely, IG2507 (7.0), IG 3984 (8.0),
FLIP2009-55L (8.3), and IG4258 (10.0) had
significantly more number of filled pods on terminal
branch. However, significantly large numbers of
unfilled pods were observed in IG3330 (37.0), IG
3364 (45.0), IG3546 (28.5), IG 4242 (29.5) and
Table 2 Mean and critical difference (CD) of heat tolerant lentil genotypes and their pollen viability
Genotype
FP/P
UFP/P
FP on TB
UFP on TB
Reduction in
100-SW (%)
Pollen viability
(%)
IG-4258
47.67
30.5
10**
3**
28.3
60.9
IG-2507
43.67
11.5
7**
2
26.2
72.4
FLIP2009-55L
43.67
19
8.25**
2
5.7
63.2
CD (P=0.05)
19.96
18.4
2.8
1.7
-
-
Note- FP/P = filled pods/plant; UFP/P =unfilled pods/plant; FP on TB= filled pods on terminal branch; UFP at TB= unfilled pods on
terminal branch
IG 4258 (30.5). Based on these observations, only
three genotypes, FLIP2009-55L, IG2507 and IG4258
were identified as heat tolerance genotypes because
these three genotypes had significantly more number
of filled pods per plant as well as on terminal branch
of each individual plant. These genotypes also had
significantly less number of unfilled pods/plant except
on IG4258 (Table 3). In order to see the impact of
late-sown conditions on seed size, data were recorded
on 100-SW under late- and normal-sown conditions.
The percent reduction in seed size observed under
late-sown condition is presented in Figure 3. It ranged
from 2.4 % to 67.2%. This was lowest in IG 4242
(2.4%) and highest in ILL 6002 (67.2%). However,
three heat tolerant genotypes FLIP2009-55L, IG2507
and IG4258 had reduction in seed size 5.7%, 26.2%
and 28.3%, respectively (Table 2). The pollen viability
was used as physiological trait and tested in laboratory
by collecting pollens at higher temperature (>35 ºC).
The pollen viability of FLIP2009-55L, IG2507 and
IG4258 was 63.2%, 72.4% and 60.9%, respectively
(Table 2). Pollen viability showed significant positive
correlation with filled pods/plant (r=0.79).
Figure 3 Effect of heat on seed size in 37 lentil accessions
Table 3 Combined ANOVA for different traits under late sown conditions (2011-12 and 2012-13)
Mean of Squares
Sources of
Variation
DF
DFF
TUFP/P
TFP/P
FP on TB
UFP on TB
100-SW
Year
1
648.12**
39.49
1179.74**
7.15
0.41
0.25
Genotype
36
68.17**
167.23*
555.67**
7.59**
1.56**
0.24**
Error
36
30.29
81.96
87.41
2.01
0.72
0.08
Heritability
(%)
38.48
8.94
46.31
58.13
36.84
50
Note: *P˂0.05, **P˂0.01
