Basic HTML Version
International Journal of Marine Science 2013, Vol.3, No.27, 212-218
http://ijms.sophiapublisher.com
217
culture media was measured as well as on the day of 7
and 14. The initial concentrations were heavy metal
concentrations in the sea waters added with treatment
concentration. These initial concentrations had used
for the following calculation, and mention as 1, 3, or 5
mg/L of heavy metals treatment. However, the initial
heavy metals of the cell concentration do not measure.
It was assume that there were no heavy metals
concentration in the initial cell, caused of the stock
had been collected from under control nutrients
and environments condition. For further research, the
measurement of initial cell concentration will provide
more detail data that able to be compared.
Algae cultured in the Walne medium without heavy
metals served as controls. All experiments were
performed in triplicates. Every day the population was
counted for 14 days. In the beginning, day of 8 and
end of experiment the concentration of Pb, Cd, Cu,
and Cr was measured with AAS.
A reduction of heavy metals was calculated as well as
P. cruentum
population. Bioconcentration Factor
(BCF) was calculated to determine the
accumulation of heavy metals in the
P. cruentum
. BCF
is a comparison between chemical concentrations on
the organism with the concentration on the environment
(Ivanciuc et al., 2006).
BCF =C
org
/C
media.
C
org
was heavy metals concentration in
Porphyridium
cruentum
(S.F. Gray) Nägeli
C
media
was heavy metals concentration in the culture
media
Author’s Contribution
Authors worked together in the laboratory experiment. TRS was responsible on
the
P. cruentum
population growth and RH for water quality control. TRS drafted
the manuscript, and authors read approved the final manuscript.
Acknowledgement
This project is supported by Indonesian Higher Education through 2012 DIPA
UNDIP for Fundamental Research Grant Number: 117b-3/UN7.5/PG/2012 16
February 2012. Thanks for Kenanga Sari and Her Nur Yoga for helping in
experimental preparation and data management.
References
Abu Al-Rub F.A., El-Naas M.H., Ashour I., and Al Marzouqi M., 2006,
Biosorption of Copper on
Chlorella vulgaris
from Single, Binary and
ternary Metal Aqueous Solutions, Process Biochemistry, 41: 457-464
http://dx.doi.org/10.1016/j.procbio.2005.07.018
Arad S.M., and Yaron A., 1992, Natural pigments from red microalgae for
use in foods and cosmetics. Trends in Food Science & Technology
,
3:
92-97
http://dx.doi.org/10.1016/0924-2244(92)90145-M
Arnot J.A. and Gobas F.A.P.C., 2006, A Review of Bioconcentration Factor
(BCF) and Bioaccumulation Factor (BAF) Assessments for Organic
Chemicals in Aquatic Organisms Environmental Reviews, 14: 257-297
Banfalvi G. 2011. Cellular effects of heavy metals. Springer. London, pp.
364
http://dx.doi.org/10.1007/978-94-007-0428-2
Belokobylsky A.I., Ginturi E.I., Kuchava N.E., Kirkesali E.I., Mosulishvili
,
L., Frontasyeva
,
M.V., Pavlov
,
M.V., and Aksenova N.G., 2004,
Accumulation of selenium and chromium in the growth dynamics of
Spirulina platensis,
Journal of Radioanalytical and Nuclear Chemistry
259(1): 65-68
http://dx.doi.org/10.1023/B:JRNC.0000015807.53132.c0
Bahar M.M.; Megharaj M. and Naidu R., 2012, Toxicity, transformation and
accumulation of inorganic arsenic species in a microalga
Scenedesmus
sp. Isolated from soil. Journal of Applied Phycology, 25: 913-917
http://dx.doi.org/10.1007/s10811-012-9923-0
Brinza L., Dring M.J., and Gavrilescu M., 2007, Marine micro- and
macro-algal species as biosorbent for heavy metals, Environmental
Engineering Management Journal 6: 237-251
Chen J.Z., Tao X.C., Xu J., Zhang T., and Liu Z.L., 2005, Biosorption of
lead, cadmium and mercury by immobilized
Microcystis aeruginosa
in
a column, Process Biochemistry 40 (12): 3675-3679
http://dx.doi.org/10.1016/j.procbio.2005.03.066
Chojnacka K., Chojnacka A., and Gorecka H., 2005, Biosorption of Cr
3+
,
Cd
2+
and Cu
2+
ions by blue–green algae
Spirulina
sp.: kinetics,
equilibrium and the mechanism of the process, Chemosphere, 59:75-84
http://dx.doi.org/10.1016/j.chemosphere.2004.10.005
Chojnacka K., 2009, Biosorption and Bioaccumulation in Practice, Nova
Science Publishers, Inc. New York, pp.149
Conti M.E., and Cecchetti G., 2003, A biomonitoring study: Trace metals
in algae and mollusks from Tyrrhenian coastal areas. Environmental
Research, 93 (1): 99-112
http://dx.doi.org/10.1016/S0013-9351(03)00012-4
Costa A.C.A., and Franca F.P., 2003, Cadmium Interaction with Microalgal
Cells, Cyanobacterial Cells, and Seaweeds; Toxicology and
Biotechnological Potential for Wastewater Treatment, Marine
Biotechnology 5: 149-156
http://dx.doi.org/10.1007/s10126-002-0109-7
Crawford R.L., and Crawford D.L., 2005, Bioremediation: principles and
applications, Cambridge University Press, New York, pp. 406
Dwivedi S., 2012, Bioremediation of heavy metal by algae: current and
future perspective. Journal of Advance Laboratory Research in Biology,
3(3): 229-233
Mustafa E.M., Phang S.M, and Chu W.L., 2012, Use of an algal consortium
of five algae in the treatment of landfill leachate using the high-rate
algal pond system, Journal of Applied Phycology 24: 953-963
http://dx.doi.org/10.1007/s10811-011-9716-x
Fuentes M.M.R., Fernandez G.G.A., Perez, J.A.S. and Guerrero J.L.G., 2000,
Biomass nutrient profiles of the microalga
Porphyridium cruentum
,
Food Chemistry 70: 345-353
http://dx.doi.org/10.1016/S0308-8146(00)00101-1
Girard J., 2010, Principles of environmental chemistry. 2nd ed. Jones and
Bartlett Publishers, LLC, pp. 677
Huang J., Chen B., and You W., 2005, Studies on Separation of Extracellular
Polysaccharide of
Porphyridium Cruentum
and its anti-HBV
in vitro
,
Chine Journal of Marine Drugs
,
24(5): 18-21
Huleihel M., Ishanu V., Tal J., and Arad S., 2011, Antiviral effect of
microalgal polysaccharides on
Herpes simplex
and
Varicella zoster
viruses.
Journal of Applied Phycology 13(2):127-134
http://dx.doi.org/10.1023/A:1011178225912
Imani S., Rezaei-Zarchi S., Hashemi M., Borna H., Javid A., Zand A.M. and
Abarghouei, H.B., 2011, Hg, Cd and Pb heavy metal bioremediation by
Dunaliella
alga,
Journal of Medicinal Plants Research 5(13):
2775-2780
Ivanciuc T., Ivanciuc O., and Klein D. J., 2006, Modeling The
Bioconcentration Factors and Bioaccumulation Factors of