International Journal of Marine Science, 2017, Vol.7, No.14, 125-129
125
Research Report Open Access
Antibacterial Activity of the Crude Extract of a Gastropod
Cellana radiata
(Born,
1778) from the Visakhapatnam Coast, Andhra Pradesh, India
Sunil Kumar Duddu, Teja Gurugubelli, Krishna Geetha Gandham, Amarnath Dogiparti
Department of Marine Living Resources, Andhra University, Visakhapatnam, India
Corresponding email
International Journal of Marine Science, 2017, Vol.7, No. 14 doi
Received: 23 Mar., 2017
Accepted: 26 Apr., 2017
Published: 08 May, 2017
Copyright © 2017
Duddu et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Duddu S.K., Gurugubelli T., Gandham K.G., and Dogiparti A., 2017, Antibacterial activity of the crude extract of a gastropod
Cellana radiata
(Born, 1778)
from the Visakhapatnam coast, Andhra Pradesh, India, International Journal of Marine Science, 7(14): 125-129 (doi
Abstract
The present study was carried out to evaluate the antibacterial activity of crude extracts of
Cellana radiata
collected from
Visakhapatnam coast, Andhra Pradesh. The whole animal extracts from methanol and ethyl acetate were obtained by both dry and
wet methods and tested for their activity against three pathogenic bacteria
i.e
.
Escherichia coli
,
Staphylococcus aureus
and
Vibrio
harveyi
. The extracts obtained from methanol (wet), ethyl acetate (dry) and ethyl acetate (wet) showed inhibition zones of 12 mm, 13
mm and 18 mm respectively against
V. harveyi
.
Keywords
Cellana radiata
; Antibacterial activity; Visakhapatnam coast
Introduction
Molluscs are soft bodied animals and play a major role among marine invertebrate fauna. The marine organisms
are exposed to complex ecosystem, tolerating wide range of fluctuations and also defence more than terrestrial
animals. So, apart from their use as food, they are also useful as bio indicators and for extracting drugs against
several diseases (Whittle, 2009; Tosti and Gallo, 2012). Some marine invertebrates which lack physical defence
will be producing certain toxic substances against to the predators for self protection and it contains some
compounds will show anti-inflammatory, antimicrobial, cytotoxic and anti-tumour properties (Burkholder and
Burkholder, 1958; McCaffrey and Endean, 1985; Koh, 1997; Anand and Edward, 2001; Mayer and Gustafson,
2006). The first attempt was carried out on marine organisms for their antimicrobial activity in 1950’s. Since then
large number of marine organisms from a wide range of phyla have been screened for their antimicrobial activity
(Shaw et al., 1976). Among the invertebrates, the molluscs are a good source for bio-medically important products
(Shenoy, 1998). Many of the molluscs have chemical defence mechanisms and produce secondary metabolites
which possess antimicrobial activities (Benkendorff et al., 2001). The bioactive compounds extracted from
molluscs were exhibiting antitumor, anti leukemic, antibacterial and antiviral activity (Rajaganapathi et al., 2002).
In recent years, the human pathogenic organisms were developing resistance to commercial drugs (Elumalai et al.,
2011). The bioactive compounds which are derived naturally have no adverse effect and also low cost than the
synthetic drug productions (Grabley and Thiericke, 1999). Some marine gastropods and bivalves have been of
great interest to natural products chemists, yielding a diversity of chemical classes and several drug leads
currently in clinical trials (Giftson and Patterson, 2014).
Cellana radiata
is an intertidal rocky shore fouler and showing homing behavior unlike like the other temperate
limpets. (Balaparameswara and Ganapati, 1971). It
has
shown antitumor property in MTT assay (Krishnamoorthi
and Yogamoorthi, 2013). So, the present attempt has been made for screening of anti-bacterial activity from
natural sources. Because, currently most of the bacteria are rapidly increasing their resistance against the
antibiotics (Penesyan et al., 2015) by reason of they are producing the various enzymes which modify or
inactivate the antibiotics (Koch, 1981).
1 Materials and Methods
Cellana radiate
(Figure 1)
were collected from inter tidal rocky shore area at VUDA Park (17°43’20.09”N and
