International Journal of Horticulture, 2017, Vol.7, No.16, 133-137
133
Research Report
Open Access
Differential Role of 6-Benzylaminopurine in Potato Tissue Culture
Siddra Ijaz
1
, and Imran-ul-Haq
2
1 Centre of Agricultural Biochemistry and Biotechnology/US-Pak Center for Advanced Studies in Agriculture and Food Security, University of Agriculture
Faisalabad, Pakistan
2 Department of Plant Pathology, University of Agriculture Faisalabad, Pakistan
Corresponding email:
International Journal of Horticulture, 2017, Vol. 7, No. 16 doi:
Received: 05 Jun., 2017
Accepted: 30 Jun., 2017
Published: 20 Jul., 2017
Copyright
©2017 Ijaz and Haq, This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Ijaz S., and Imran-ul-Haq, 2017, Differential role of 6-Benzylaminopurine in potato tissue culture, International Journal of Horticulture, 7(16): 133-137 (doi:
)
Abstract
Phytohormones are the derivatives of secondary metabolic pathways. They are of diverse physiological role, molecular
action and metabolism but they do cross talk during hormonal signaling pathways. During
in vitro
micropropagation and
regeneration studies in potato (
Solanum tuberosum
L.), so called berries like structures were observed. In these studies the effect of
gibberellic acid (GA
3
), NAA (auxin) and BAP (cytokinins) were being investigating on
in vitro
micropropagation and
in vitro
regeneration of
Solanum tuberosum
L. (potato). In
in vitro
micropropagation study when potato tubers of cv. PRI-Red were treated
with 0.3% GA
3
to induce sprouting and these sprouts were when cultured on micropropagation media containing 1.75, 2.75 mg/l
BAP, then so called berries like structures were formed. Similarly, in
in vitro
regeneration study, when calli of potato cv. kuroda,
induced on callus induction medium containing 4.5 mg/l 2-4D were when shifted on regeneration medium containing 4.75 mg/l BAP
then same so-called berries like structures were formed. Hence histoanatomy of these berries like structure and microtubers were
done which revealed that these are anatomically entirely different from microtubers.
Keywords
Phytohormones; Potato; BAP; Gibberellic acid
1 Introduction
Plant hormones have manifold and diverse effects though conditional on plant developmental stage, hormonal
concentration as well as site of action (Liu and Chen, 2009; Jaillais and Chory, 2010). They have significant
impact on plant growth and development by affecting its cell division, cell differentiation and elongation. These
are categorized into five classes, viz., auxins, cytokinins, ethylene, abscisic acid and gibberellins (gibberellic
acid). Among these, auxins and gibberellic acid control expansion along longitudinal axes and significantly affect
size of organs and plant architecture (Liu and Chen, 2009).Therebyin this research note, differential effect of
6-benzylainopurie (BAP) on potatoes discussed.
2 Plant Material
In vitro m
icropropagation study was conducted on potato genotype, PRI-red while in vitro regeneration study was
based on genotype Kuroda.
2.1 Sprouting and preparation of explant to be used
Tubers of potato (
Solanum tuberosum
L.) cultivar, PRI-Red were used as source of explant (axillary bud of sprout)
in micropropagation. For surface sterilization, tubers were washed with water and dipped in detergent solution
containing 2-3 drops of tween-20 for 15 minutes. Thereafter, tubers were rinsed 4-5 times with autoclaved
ultra-pure water and soaked in 0.3% GA
3
solution for 15 minutes and 30 minutes. These GA
3
treated tubers were
then wrapped with paper bag and kept in dark condition at 26±1˚C for sprouting. After sprouting the tubers,
explant was surface sterilized by soaking in ultra-pure water and also in detergent solution containing 2-3 drops
of tween-20 for few minutes. After that, sprouted tubers were rinsed 5-6 times with ultra-pure water. Subsequent
sterilization operation was done in axenic condition under laminar air flow cabinet. Sprouts were cut into small
section containing 1-2 buds each followed by dipping in 70% ethanol for 1 minute and 0.1 HgCl
2
solution for 5
minutes. Thereafter explants were rinsed 4-5 times with ultra-pure water.
