International Journal of Aquaculture, 2017, Vol.7, No.9, 64-70
67
selenium (2.5 mg/l) exposed to
Channa punctatus
(Blouch) (Avinashe and Patil, 2011). 96 hours LC
50
of 4.32mg/l
was also recorded when
Oreochromis
species was exposed to selenium concentration (Hossam et al., 2009) The
mortality recorded in the study might be a consequence of stress induced by selenium on the immune system of
C.
gariepinus
which probably slow toxic progress and resulted in acute toxic response.
Table 1 Haematological parameters of
C. gariepinus
Parameter
TRT 1
TRT 2
TRT 3
TRT 4
TRT 5
CONTROL
PCV (%V)
22.67±1.15
d
21.67±0.58
d
19.00±1.00
c
17.00±0.50
b
12.50±0.55
a
48.33± 0.58
e
WBC(x10
3
mm
3
) 1816.67±76.38
b
1783.33±28.86
b
1440.00±69.28
a
1536.67±124.23
a
1410.00±75.50
a
2470.00±26.46
c
HB (x10
2
g/dl)
7.93±0.47
c
7.37±0.06
c
6.21±0.11
b
5.00±0.46
a
4.96±0.83
a
25.71±0.34
d
RBC (x10
7
/µl)
2.95±0.16
d
2.81±0.06
d
2.47±0.13
c
1.31±0.02
b
1.02±0.01
a
6.29±0.07
e
MCV (x10
-7
Fl) 76.53±0.47
a
76.88±0.46
a
76.92±0.00
a
128.08±3.02
c
122.52±3.70
b
76.80±0.00
a
MCH (x10
-7
pg) 27.72±1.04
a
26.16±0.67
a
25.15±0.89
a
37.97±3.50
b
46.68±8.33
c
40.84±0.76
b
MCHC (pg)
36.07±1.29
bc
34.02±1.07
abc
32.71±1.15
ab
29.64±2.16
a
39.81±7.26
c
53.23±1.06
cd
Note: Mean with different superscripts are significantly different
Table 2 Water quality parameters of culture system of
C. gariepinus
observed over period of 96 hours
Parameters
Control (0 mg/l) TRT 1 (2 mg/l) TRT 2 (3 mg/l) TRT 3 (4 mg/l) TRT 4 (5 mg/l) TRT 5 (6 mg/l)
TEMP(OC)
25.53±0.13
ab
25.53±0.09
ab
25.48±0.16
a
25.68±0.68
b
25.48±0.10
a
25.50±0.18
a
pH
6.58±0.16
a
6.48±0.15
a
6.44±0.12
a
6.40±0.07
a
6.44±0.10
a
6.47±0.10
a
D.O (ppm)
4.11±0.22
e
3.08±0.17
d
2.99±0.18
d
2.75±0.28
c
2.27±0.20
b
1.52±0.34
a
SAL (ppm)
0.08±0.00
a
0.08±0.00
a
0.08±0.00
a
0.08±0.00
a
0.08±0.00
a
0.08±0.00
a
COND(mn-cm)
0.0042±0.00
a
0.0043±0.00
a
0.0042±0.00
a
0.0042±0.00
a
0.0043±0.00
a
0.0042±0.00
a
T.D.S (ppm)
95.83±1.10
a
96.00±1.75
a
95.67±2.31
a
95.17±1.80
a
95.17±1.11
a
95.08±1.93
a
Ammonia(ppm)
0.4±0.16
a
0.34±0.15
a
0.33±0.15
a
0.35±0.17
a
0.40±0.15
a
0.33±0.15
a
Nitrate(ppm)
7.08±2.57
a
7.50±2.61
a
6.67±2.46
a
7.50±2.46
a
6.67±2.46
a
7.08±2.57
a
Nitrite(ppm)
1.42±0.51
a
1.33±0.49
a
1.42±0.51
a
1.33±0.49
a
1.42±0.51
a
1.41±0.51
a
Note: Mean with different superscripts are significantly different
Figure 2 96 hours LC
50
of
Clarias gariepinus
juvenile exposed to selenium
Blood parameters have been used as sensitive indicator of stress in fish exposed to different water pollutants and
toxicants, such as metals, biocides, pesticides and industrial effluents (Dharam et al., 2008). The study of
