International Journal of Aquaculture, 2016, Vol.6, No.8, 1-10
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Table 3 Effect of fungicide (
bavistin
) treatment on culture establishment of
L. antipoda
(L.) nodal explants
SI. No
Treatments
Rate of Contamination
Rate of survival after 15 days Percentage of bud sprouting
1.
T6-Bavistin (0.15%)
15.5%
84.5%
80%
2.
T7- Bavistin (0.3%)
6%
75%
48%
Murashige and Skoog (1962), one of the widely used plant tissue culture media, was used for present study. For
shoot initiation from explants two different ionic strength of MS media were used, Full strength MS (Full MS) and
half strength MS (Half MS) (Figure 1). In full MS medium, the growth rate of axillary buds was about 55.6%
while in ½ MS media it was 95.6% (Figure 2). Here, half of the concentration of MS medium was taken, because
it was observed that, the half strength MS medium favored better growth response and axillary shoot proliferation
than full strength MS medium.
Figure 1: Culture initiation of
L. antipoda
L. on ½ MS media
basal media
Figure 2: Culture establishment of
L. antipoda
L.
on ½ MS
media + 1mg/l BAP after 4 weeks of culture initiation
After culture establishment, plants multiplied in different culture media. The semisolid and liquid culture media of
MS and ½ MS media was used. Highest multiplication was obtained on half strength MS liquid media (Figure 3).
The response of
L. antipoda
(L.) on solid and liquid culture media having different ionic strength is explained in
Table 4.
Figure 3: Multiple shoot proliferation of
L. antipoda
L. on BM
3
media after 2 weeks of culture initiation
