Genomics and Applied Biology 2018, Vol.9, No.2, 6-13
6
Research Article Open Access
The Influence of GA on the Germination of
Sophora davidii
Jie Liu
1, 2
, Yufang Zhuang
1
, Ting Chen
1
, Weijia Xie
3
1 School of Life Science, Guizhou Normal University, Guiyang, 550001, China
2 Key Laboratory of Plant Physiology and Development Regulation, Guizhou Province, Guiyang, 550001, China
3 Institute of Flowers, Yunnan Academy of Agricultural Sciences, Yunnan, 650051, China
Corresponding author email:
Genomics and Applied Biology, 2018, Vol.9, No.2 doi:
10.5376/gab.2018.09.0002
Received: 16 Mar., 2018
Accepted: 27 Mar., 2018
Published: 04 May, 2018
Copyright © 2018
Liu et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Liu J., Zhuang Y.F., Chen T., and Xie W.J., 2018, The influence of GA on the germination of
Sophora davidii
, Genomics and Applied Biology, 9(2): 6-13
(doi:
10.5376/gab.2018.09.0002
)
Abstract
The seed of
Sophora davidii
is a high-activity hard seed, of which the seed coat is thick and tough, limiting the
penetration of water and exchange of gas in embryo, thereby inhibiting the respiration of seeds and mechanically restraining the
growth of embryo. If not treated, the germination rate would be very low. In this study, the
Sophora davidii
seeds were treated by
gibberellin (GA) with different concentrations (0 mg/L, 50 mg/L, 100 mg/L, 200 mg/L, 300 m/L, 500 mg/L) for different times. Then,
we measured the quality, length, width, thickness, testa thickness, water absorption sites, germination rate, root length and other
physiological and biochemical indexes of seeds, so as to discuss the effect of GA on seed germination and root formation condition
of
Sophora davidii
. The result showed that seed of
Sophora davidii
had thick and hard coat, and its water absorption sites were not
only on hilum. After polishing, seeds treated with 200 mg/L GA3 had the highest germination rate, which reached 95%. Also, its root
growth was the longest, reaching 0.05 cm.
Keywords
Sophora davidii
; Water absorption sites; GA3; Seed germination rate; Root growth
Background
Sophora davidii
, also known as Tiemashao, Langyaci, Kuci, (Names of Sophora davidii in Chinese) Leguminosae
papillionoideae sophora, and bush, widely distributes in the provinces of Hubei, Gansu, Shaanxi, Guizhou,
Yunnan, Sichuan and Jiangsu of China. It could be a pioneer tree species for protecting water and soil excellently,
recovering vegetation, and improving soil (The Institute of Botany, Chinese Academy of Sciences, 1981; Xu, 1997;
Chen, 2002; Li et al., 2010).
The seed dormancy usually refers to the phenomenon that a viable seed can’t germinate normally under suitable
conditions. It is a crucial characteristic of seed plants to adapt to environment changes and ensure seed
germination at the most suitable time. It would be great significance to maintain the continuity of species. Current
research showed several reasons of seeds dormancy: (1) the limitation of seed coat, which was airtight and
impervious, or the seed coat was too thick; (2) the seeds that unfinished after-ripening needed to be treated with
low temperature; (3) embryo was not fully developed; (4) the existence of inhibiting substances (Bewley, 1997;
Yang et al., 2003; Tang et al., 2004; Pan et al., 2006; Pan and Xu, 2010). The treatments of hard seeds include
three methods: physical method, chemical method and biological method. The physical methods include scraping
seed coat, high temperature treatment, low temperature treatment, etc.; the chemical methods include acid etching,
lye, hormone, etc.; the biological methods include the slow function of microorganism in nature, etc. (Li et al.,
2010). At present, the research on seed coat treatment and its effect on seed germination rate had been carried out
extensively. Among them, the authors of the passage: Study on the Properties of Hard and Germination of
SophoraViciifolia
Seed (Guo et al., 2010) dealt with the seeds of
Sophora davidii
by different methods. The
research showed that the best method was breaking in seed coat by machinery, and the worst method was treated
by hot water.
