Genomics and Applied Biology 2019, Vol.10, No.1, 1-9
8
combining the software of miREvo (Wen et al., 2012) and mirdeep2 (Friedlander et al., 2012). Familial analysis of
the obtained new miRNA and known miRNA was carried out to explore the presence of miRNA family of tea
plants in other species.
3.5 Target gene prediction and functional annotation
The known miRNA and new miRNA obtained from the analysis were predicted by using the software psRobot.
According to the corresponding relation between the miRNA and its target genes, gene ontology analysis was
conducted on the website (
/) and pathway significant enrichment analysis was
conducted on the website of Kyoto Encyclopedia of Genes and Genomes database, respectively.
Authors’ contributions
SWX, XLF is the main executor of the experimental design and experimental research of this research, complete the experimental
data analysis and the writing of the first draft of the thesis; TYP, JHB, SYN, LDH participated in the experiment design, the
experiment result analysis; CLB is the project’s architect and director, guiding experimental design, data analysis, paper writing and
revision. All authors read and approved the final manuscript.
Acknowledgments
This study was co-funded by the National Natural Science Foundation of China (31560220), the State key Laboratory of Tea Biology
and Resource Utilization (SKLTOF20150105) and the Yunnan Province Talent training Program (20-15HB105).
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