Page 6 - Molecular Plant Breeding

Basic HTML Version

Molecular Plant Breeding 2013, Vol.5, No.9, 47
-
63
http://mpb.biopublisher.ca
49
Figure 1 Distribution of salt tolerance score (SES), in 300
F
5
–derived recombinant inbred lines from IR29×Hasawi
Note: Mean values (indicated in arrows), were 7 for IR 29 (P1),
4 for Hasawi (P2), and 6 for the RILs (M), A value of 4 or
below indicates tolerance
Figure 2 Distribution of plant height under saline stress in 300
F
5
–derived recombinant inbred lines from IR29×Hasawi
Note: Mean values (indicated in arrows) were 17 cm for IR 29
(P1), 28 cm for Hasawi (P2) and 21.4 cm for the RILs (M)
Figure 3 Distribution of root length under saline stress in 300
F
5
–derived recombinant inbred lines from IR29×Hasawi
Note: Mean values (indicated in arrows) were 8 cm for IR 29
(P1), 10 cm for Hasawi (P2) and 10.2 cm for the RILs (M)
Figure 4 Distribution of fresh weight under saline stress in 300
F
5
–derived recombinant inbred lines from IR29×Hasawi
Note: Mean values (indicated in arrows) were 0.144 g for IR 29
(P1), 0.352 g for Hasawi (P2) and 0.26 g for the RILs (M)
range
of
variation
SES
score
of
3~9
(Table
1;
Figure
1),
Thirty-one
31,
RILs
had
tolerance
level
similar
or
better
than
Hasawi
as
determined
by
their
SES
score
of
4
or
less.
Plant
height:
The
percent
mean
reduction
of
plant
height
under
saline
conditions
relative
to
non-saline
condition
was
relatively
lower
in
the
susceptible
parent
IR29
11.76%,
than
the
average
of
the
F
5
RILs
15.63%, A
significant
range was
observed
in
the RILs
for
plant
height
(Table
1;
Figure
2),
Some
RIL
had
higher
plant
height
than
the
tolerant
parent
under
both
saline
stress
44
cm
for
RILs
vs.
28
cm
for
Hasawi,
and
in
the
non-stress
replication
46
cm
for
RILs
vs.
29
cm
for
Hasawi,
Table
1,
Compared
to
the
control
condition;
salinity
had
a
profound
effect
on
the
plant
height
causing
a
mean
reduction
of
16%
among
the
RILs.
Root
length.
Salinity
caused
significant
reduction
in
root
length
for
the
RILs
(Table
1),
Reduction
in
root
length
due
to
salinity
was
33%
for
IR29,
while
the
average
reduction
for
root
length
among
F
5
RILs was
20%. The mean
root
length
observed
under
non
saline
controls
was
16.68cm
and
10.15cm
under
saline
conditions.
Of
the
300
RILs
evaluated,
86
representing
29%
of
the
RILs
had
an
increase
of
up
to
15
%
in
root
length
over
Hasawi;
the
donor
and
tolerant
parent
(Figure
3),
whilst
the
average
effect
of
salinity
on
root
length
was
20%
relative
to
the
control
trials.
1.2 Shoot
fresh
weight
The
mean
shoot
fresh
weight
observed
among
the
RILs was
0.26
g
ranging
from
0.04
g
to
0.05
g
under