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Molecular Plant Breeding 2013, Vol.5, No.3, 10
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Ib is the band informativeness. Ib is calculated by
using the formula, Ib=1-[2×I0.5-pI], where p is the
proportion of genotypes containing the band (Salazar
et al., 2007). Marker Index for all the 15 primers was
calculated according to Powell et al., (1996), which
was further used by Milbourne et al., 1997. Marker
Index=DI×EMR, where DI is the Diversity index that
is equivalent to PIC and EMR is the effective
multiplex ratio and EMR=fraction of polymorphic
loci×number of polymorphic loci. Finally the cluster
analysis was done, to determine genetic diversity.
Binary data matrix was then used to construct a
dendogram based on UPGMA (unweighted pair group
method of arithmetic averages) using STATISTICA
software, version 7.
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