Basic HTML Version
Molecular Plant Breeding 2011, Vol.2, No.07, 41
-
47
http://mpb.sophiapublisher.com
43
Table 1 Amplification results of 25 AFLP primer combinations in 30 Caixins
Serial No. Primer combination
Total no. bands Polymorphic bands
Percentage of Polymorphic loci (%)
PIC
1
E-AAC
M-CAC
63
54
85.7%
0.031 2
2
E-AAC
M-CGT
44
25
56.8%
0.011 6
3
E-AAA
M-CAC
55
51
92.7%
0.022 1
4
E-AAA
M-CAG 84
69
82.1%
0.021 9
5
E-AAA
M-CTC
32
23
71.9%
0.020 2
6
E-AAA
M-CTG
50
36
72%
0.024 9
7
E-AAA
M-CCT
45
41
91.1%
0.023 3
8
E-ATG
M-CAC
44
38
86.4%
0.021 8
9
E-ATG
M-CAG 42
31
73.8%
0.016 7
10
E-ATG
M-CTC
48
29
60.4%
0.015 6
11
E-ATG
M-CTG
35
34
97.1%
0.025 5
12
E-ATG
M-CCG
44
37
84.1%
0.031 7
13
E-ATG
M-CGT
84
58
69.1%
0.021 5
14
E-ACT
M-CTC
13
10
76.9%
0.016
15
E-ACT
M-CCG
26
18
69.2%
0.030 5
16
E-ACT
M-CGA 33
25
75.8%
0.030 3
17
E-ACT
M-CGT
38
32
84.2%
0.020 6
18
E-ACG
M-CAC
31
21
67.7%
0.030 8
19
E-ACG
M-CAG 33
30
90.9%
0.025 3
20
E-ACG
M-CTC
46
39
84.8%
0.028 7
21
E-ACG
M-CTG
52
49
94.2%
0.021 3
22
E-ACG
M-CCT
22
18
81.8%
0.024 4
23
E-AGC
M-CAG 32
27
84.4%
0.022 7
24
E-AGC
M-CTG
58
49
84.5%
0.027 8
25
E-AGC
M-CGT
34
28
82.4%
0.031 3
Note: Core sequence: E-: GACTGCGTACCAATTC; M-: GATGAGTCCTGAGTA; PIC: polymorphism information content
Table 2 The parameters of genetic diversity of 30 caixins based on AFLP data
Name
%P
Na
Ne
I
He
Name
%P
Na
Ne
I
He
Dong Guan 45 Days
87.21% 1.763 1.524 0.47
0.358 Lvbao 701
51.60% 1.032 1.211 0.232 0.165
Teqing 60 Days
86.76% 1.836 1.604 0.501 0.39
GX3
-
1
89.95% 1.877 1.637 0.523 0.409
Australia 008
85.39% 1.836 1.635 0.512 0.403 GX3
-
6
80.37% 1.612 1.403 0.396 0.293
Australia 608
85.39% 1.826 1.601 0.495 0.387 GX3
-
6
-
1
89.50% 1.836 1.576 0.499 0.384
Hong Kong 45 Days
87.21% 1.785 1.524 0.468 0.356 GX5
-
3
88.13% 1.799 1.597 0.504 0.391
Youqing no. 12
86.30% 1.767 1.532 0.469 0.358 GX5
-
4
88.13% 1.781 1.535 0.477 0.363
Si Jiu
-
19
88.58% 1.845 1.626 0.52
0.407 GX5
-
12
91.32% 1.886 1.651 0.536 0.419
New Zealand Si Jiu
84.93% 1.717 1.491 0.449 0.34
GX5
-
14
84.47% 1.703 1.462 0.433 0.325
Mingyou 308 sweet
86.30% 1.753 1.518 0.465 0.353 GX6
-
6
88.58% 1.831 1.558 0.486 0.372
No. 31
87.67% 1.804 1.55
0.483 0.37
GX6
-
6
-
1
84.47% 1.799 1.632 0.508 0.4
Australia 50 Days
86.30% 1.808 1.583 0.494 0.384 GX6
-
7
89.50% 1.826 1.56
0.489 0.375
Kuaida 28 Days
88.13% 1.817 1.585 0.5
0.386 GX6
-
13
87.21% 1.763 1.544 0.477 0.365
Youxin no. 2
88.58% 1.831 1.578 0.496 0.383 GX6
-
14
82.19% 1.644 1.47
0.432 0.326
Gui liu October
91.78% 1.904 1.665 0.543 0.426 GX9
-
1
78.54% 1.575 1.436 0.407 0.306
Caixingli Si Jiu
87.21% 1.772 1.532 0.473 0.361 GX9
-
4
78.08% 1.603 1.504 0.434 0.334
Note: %P: Percentage of Polymorphic Loci; Na : No. of Different Alleles; Ne : No. of Effective Alleles; I : Shannon's Information
Index