Basic HTML Version
Huang et al., 2011, Integrating the
hrap
Gene from Sweet Pepper into Potato Enhances Resistance to
Phytopthora infestans
, Molecular Plant Breeding Vol.2
No.5 (doi: 10.5376/mpb.2011.02.0005)
36
3.7.2 Identification of bacterial wilt
Bacterial wilt strain TB48 was prepared on a
triphenyltetrazolium chloride (TTC) plate, inoculated
into TTC medium and cultivated at room temperature
in a shaking incubator at 150 rpm for use. The
prepared bacilli were added to a culture bottle
containing MS medium (
OD
520
=0.2). Different strains
of potato plants were placed into the bottle, which was
maintained in a greenhouse with daily observations.
Authors’ contributions
JMH, LL and DYP carried out the trait of transformation, PCR and DNA
extraction. CJ worked on the vector construction and Southern Blot analysis.
ZZY worked on RT-PCR and DNA sequencing. LZP participated in the
design of experiment and the modification of the manuscript. HXQ
conceived the overall study, performed the experiment designs and took part
in the data analysis as well as the preparation of the manuscript. All the
authors have read and approved the final manuscript.
Acknowledgements
This study was jointly supported by the Scientific Research Foundation of
Ministry of Education, the Projects for Professional Talents funded by
Personnel Department of Guizhou Province, the Scientific Foundation of
Guizhoug Province (No. 2006-2032), Programs Foundation for Ph.D of
Guizhou Agricultural Academy and the Scientific Planning Project of Fujian
Province (2003N085).
We thank Prof. Teng-Yung Feng (Institute of Botany, Academia Sinica,
Taipei, Taiwan) for kindly providing
hrap
gene. We also thank Prof. Yan-Li
Yang (Department of Plant Protection, Yunnan University) for identifying
the resistance of the transgenic plants.
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