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Legume Genomics and Genetics (online), 2010, Vol. 1, No.4, 18-23
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22
part over leaves (Nisar et al., 2006). Two isogenic lines
Falloner (powdery mildew resistant) and 11760
-
3ER
(powdery mildew susceptible) having six morpho-
logical contrasting traits i.e. anthocyanin pigmentation,
flower colour, pod colour, tendril type, leaf type and
seed texture were selected and cross was made to
ensure the inheritance and association (linkages) of
the traits (Table 4).
Table 4 Six contrasting traits among the selected parents of
Pisum sativum
Parents
No.
Contrasting Traits
Fallon (Parent -♀)
11760-3 (Parent - ♂)
1
Anthocyanin Pigmentation
Pigmentation absent
Pigmentation present
2
Flower colour
Creamy
Purple
3
Pod colour
Green
Purple
4
Tendril
Bushy
Normal
5
Number of leaflets
Leafless
Leaflet present
6
Seed texture
Smooth
Rough
7
Disease
Resistant
Susceptible
3.2 Inheritance qualitative traits
F
1
seeds (♀Fallon×11760
-
3♂) were sown in the screen
house of IABGR, NARC during 2005~2006 to get the
F
2
seeds. To determine the inheritance of powdery
mildew and its association with other qualitative traits,
parents along with F
1
and F
2
seeds were sown in the
screen house of IABGR, NARC during 2006~2007.
Optimum conditions were provided for the growth of
fungus
Erysiphe pisi
; however, the resistant plants
were artificially inoculated with conidia of the fungus
by dusting from the leaves. The data were recorded on
46 F
2
segregates on individual plant basis.
3.3 Inheritance of quantitative traits
To select high yielding powdery mildew resistant
genotype, check varieties DASAN was also sown for
comparison. The F
2
generation was sown during
2007~2008 in the same condition and recommended
procedures used for F
1
generation to get F
3
generation.
In F
3
generation, four yield contributing quantitative
traits; total pod weight, twenty seed weight, pod length
and number of seeds pods were studied in 97 plants.
3.4 Data analysis
The inheritance and association of various qualitative
and quantitative traits in F
2
and F
3
generation were sub-
jected to different statistical analysis. The data scored
on qualitative traits (F
2
) were analyzed through chi-
square test and likelihood ratios, while that of quantitative
traits (F
3
) were subjected to frequency distribution, coe-
fficient of variation and correlation coefficient. Based
on total pod weight and powdery mildew sensitivity high
yielding powdery mildew resistant lines were selected.
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