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Legume Genomics and Genetics (online), 2010, Vol. 1, No.1, 1-6
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the germplasm from European and Mediterranean by
RAPD technique (Link et al., 1996). The variation of
twenty-six faba bean populations from five geogra-
phic areas and five varietal types have been revealed
by seven isozymes that the results supported the hypo-
thesis of an isolating origination of the crop in Ethio-
pia and in Asian (Serradilla et al., 1993). Three isozy-
mes, glutamate oxaloacetate transaminase (GOT), su-
peroxide dismutase (SOD), and malic enzyme (ME),
had been analysed electrophoretically to study the di-
versity within and between 33 faba bean (
V. faba
L.
type minor) accessions, which were grouped as five
groups with significant differences among them based
on the parameters of principal components, hierar-
chical cluster, and canonical discriminant analyses
(Polignano et al., 1998). Core collection of broad bean
in Qinghai was preliminary constructed based on mor-
phological traits as well (Yang et al., 2009).
In this research we analyzed the genetic diversities of
faba bean germplasms collected in Qinghai using AF-
LP method and constructed core faba germplasm co-
llection based the data of genetic diversity. It might
provide new insight for understanding the genetic
basis of Qinghai faba bean germplasm as well as for
better using the germplasm in breeding program.
1 Results
1.1 The polymorphism of AFLP primer analyzed
Ten pairs of primer combination can generated poly-
morphic bands in the ranges from 3 to 10 bands res-
pectively, the primers combination named as E1/M6,
E2/M2, E4/M4 and E7/M7 generated ten polymorphic
bands and their polymorphic fragment ratio reached
52.63%, 52.63%, 50.00% and 52.63%, respectively,
accordingly the proportion of effective alleles to all-
eles were respectively 67.80%, 68.46%, 73.35% and
68.59%. The pair of primer of E6/M6 produced three
polymorphic bands only; Seven to eight polymorphic
bands could be amplified from the rest primers com-
bination (Table 1).
1.2 The AFLP polymorphism of faba bean collected
in Qinghai
The average effective alleles were about 1.064 7 among
149 tested faba bean collections detected by using ten
pairs of primers, which the proportion of effective all-
eles to alleles was 72.52%, as well as the polymorphic
fragment ratio was 46.82% based on the total 81 am-
plified polymorphic bands, further Shannon’s infor-
mation index was 0.094 6. The proportions of effe-
ctive alleles to alleles were detected as 90.39%, 81.76%
and 72.52% for released cultivars, introduced acces-
sions and foreign germplasms, respectively. The poly-
morphism of local varieties were much higher with
the 44.51% polymorphic fragment radio and 0.051 2
Nei’s gene diversity, whereas the one of released
varieties was quite lower with the polymorphic frag-
ment ratio and Nei’s gene diversity, which was 17.92%
and 0.045 5 (Table 2).
1.3 Genetic structure grouping analysis
Genetic Structure of tested faba bean germplasms
were analyzed by the software structure 2.2. The fig-
ure of K point of inflection was drawn based on the
results of analysis (Figure 1). There was the first ob-
vious inflexion when K value was up to seven, which
imply that the optimum grouping of tested faba bean
in this research should be divided into seven groups
(Figure 2).
Table 1 The polymorphic index for tested the AFLP primer combinations
Primer Number of
alleles (na)
Number of effective
alleles (ne)
ne/na
Poly-band Poly-ratio Shannon's
information index
Gene diversity
E1/M1 1.388 9±0.501 6
1.092 7±0.176 6
78.67
7
38.90
0.112 0±0.190 8
0.066 6±0.120 3
E1/M6 1.526 3±0.513 0
1.034 9±0.052 4
67.80
10
52.63
0.069 4±0.087 9
0.031 5±0.044 9
E2/M2 1.526 3±0.513 0
1.044 9±0.063 5
68.46
10
52.63
0.082 7±0.106 3
0.039 8±0.055 0
E4/M4 1.500 0±0.513 0
1.100 2±0.184 1
73.35
10
50.00
0.130 7±0.177 0
0.073 3±0.113 8
E5/M2 1.500 0±0.518 9
1.040 1±0.072 9
69.34
7
50.00
0.071 6±0.108 4
0.034 7±0.059 4
E5/M5 1.388 9±0.501 6
1.085 1±0.158 3
78.13
7
38.89
0.108 9±0.180 1
0.063 0±0.111 7
E6/M4 1.470 6±0.514 5
1.056 7±0.113 1
71.86
8
47.06
0.086 5±0.138 3
0.045 5±0.082 3
E6/M6 1.250 0±0.452 3
1.021 0±0.038 4
81.68
3
25.00
0.042 1±0.076 7
0.019 3±0.035 3
E7/M7 1.526 3±0.513 0
1.046 9±0.093 0
68.59
10
52.63
0.078 5±0.119 0
0.039 0±0.069 5
E8/M8 1.529 4±0.514 5
1.106 1±0.169 5
72.32
9
52.94
0.142 2±0.181 1
0.080 1±0.112 8